Table 1

Primers used for the reverse transcriptase PCR

Gene

Primer Sequence

Length

Tm (oC)

Product size


CRALBP

forward

AGA ACT CTC CAG CTT CTA CCA GGA

24 bp

60.2

301 bp

reverse

AGC TTG GGA GGA GGA TGA CAT CTG

24 bp

59.8


β-Actin

forward

TCA TGA TCG AGA CCT TCA ACA CCC

24 bp

60.3

288 bp

reverse

AGC AGA GCT TCT CCT TGA TGT CAC

24 bp

60.1


RPE-65

forward

CCA GGG CTG CTG AGG TGG TTA ACA

24 bp

60.0

278 bp

reverse

GTG CCT GTC TCA CGA AGT ACG ATT

24 bp

60.1


Vimentin

forward

ATG AAG GAA GAG ATG GCT CGT CAC

24 bp

59.9

173 bp

reverse

TCC AGA TTG GTT TCC CTC AGG TTC

24 bp

60.0


Primer sequences, lengths, melting temperatures and product sizes have been included used in the identification of RPE-specific genes and control genes for primary RPE cells and tRPE cell cultures.

Liggett et al. BMC Cell Biology 2009 10:33   doi:10.1186/1471-2121-10-33

Open Data