Table 1 |
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|
Primers used for the reverse transcriptase PCR |
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|
Gene |
Primer Sequence |
Length |
Tm (oC) |
Product size |
|
|
|
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|
CRALBP |
forward |
AGA ACT CTC CAG CTT CTA CCA GGA |
24 bp |
60.2 |
301 bp |
|
reverse |
AGC TTG GGA GGA GGA TGA CAT CTG |
24 bp |
59.8 |
||
|
|
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|
β-Actin |
forward |
TCA TGA TCG AGA CCT TCA ACA CCC |
24 bp |
60.3 |
288 bp |
|
reverse |
AGC AGA GCT TCT CCT TGA TGT CAC |
24 bp |
60.1 |
||
|
|
|||||
|
RPE-65 |
forward |
CCA GGG CTG CTG AGG TGG TTA ACA |
24 bp |
60.0 |
278 bp |
|
reverse |
GTG CCT GTC TCA CGA AGT ACG ATT |
24 bp |
60.1 |
||
|
|
|||||
|
Vimentin |
forward |
ATG AAG GAA GAG ATG GCT CGT CAC |
24 bp |
59.9 |
173 bp |
|
reverse |
TCC AGA TTG GTT TCC CTC AGG TTC |
24 bp |
60.0 |
||
|
|
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|
Primer sequences, lengths, melting temperatures and product sizes have been included used in the identification of RPE-specific genes and control genes for primary RPE cells and tRPE cell cultures. |
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|
Liggett et al. BMC Cell Biology 2009 10:33 doi:10.1186/1471-2121-10-33 |
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