Loss of CSN2 results in the formation of CSN5 containing CSN subcomplexes. K562 cells were transiently co-transfected with HKK plasmid together with either shVC or shCSN2 plasmid. HKK positive cells were sorted 24 hours post-transfection, re-cultured and harvested day 3 post transfection. Both CSN2 (A) and CSN5 (B, short exposure; C, long exposure) distribution in shVC and shCSN2 cells was determined by 2-Dimensional Native-PAGE/SDS-PAGE and western blot analysis. All data shown is representative of n = 3 transfections.
Pearce et al. BMC Cell Biology 2009 10:31 doi:10.1186/1471-2121-10-31