The effect of increasing concentrations of compound 1 on FGF-2 binding to FGFR-1 and -2. The soluble receptor was coated onto the wells of a 96-well plate and blocked with 1% BSA. The test compounds and growth factor were premixed for 2 h and added to the plate. After 2 h the plate was washed and incubated with antibodies to the growth factor, then peroxidase-conjugated IgG for 45 min. Peroxidase substrate was added and the absorbance read at 405 nm (se below for detail).
Hussain et al. BMC Cell Biology 2009 10:30 doi:10.1186/1471-2121-10-30