The effect of compound 1 on BAEC tube formation in Matrigel with and without FGF-2. (A) Compound 1 inhibited FGF-2 (25 ng/ml) induced BAEC differentiation into capillary like structures: column1, control. In the absence of FGF-2 there was no inhibitory effect on tube formation. Values significantly different from FGF-2 alone (p < 0.05) are shown by *. Results are the mean of three experiments (B) Representative photomicrographs (original magnification × 20) show tube formation by BAEC. In the presence of FGF-2 a closed tubular network was evident (an example is shown in II) and this was abolished by compound 1 (shown in IV). In the control (I0 and with compound 1 alone (III) there was no evidence of tube formation.
Hussain et al. BMC Cell Biology 2009 10:30 doi:10.1186/1471-2121-10-30