Figure 2.

A. Differentiation of mMSCs and GFPTgMSCs along mesodermal lineages. Cells were incubated in CEM and then transferred to adipogenic or osteogenic media for 14 days. All cells were passage 9 or lower. Cells which formed lipid vacuoles were stained with Oil Red-O while mineralization in osteogenic-differentiated cells was revealed with Alizarin Red staining.B. GFPTgMSCs retain fluorescence after differentiation. MSCs derived from eGFP+ transgenic C57Bl/6 mice (P6) retain fluorescence even after stimulated to undergo adipogenic differentiation. 20× magnification.

Ripoll and Bunnell BMC Cell Biology 2009 10:3   doi:10.1186/1471-2121-10-3
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