A. Differentiation of mMSCs and GFPTgMSCs along mesodermal lineages. Cells were incubated in CEM and then transferred to adipogenic or osteogenic media for 14 days. All cells were passage 9 or lower. Cells which formed lipid vacuoles were stained with Oil Red-O while mineralization in osteogenic-differentiated cells was revealed with Alizarin Red staining.B. GFPTgMSCs retain fluorescence after differentiation. MSCs derived from eGFP+ transgenic C57Bl/6 mice (P6) retain fluorescence even after stimulated to undergo adipogenic differentiation. 20× magnification.
Ripoll and Bunnell BMC Cell Biology 2009 10:3 doi:10.1186/1471-2121-10-3