BM-derived MSCs express stem cell marker protein Oct4. Cells (5th passage) were fixed and incubated with antibodies directed against Oct4. Immunoreactivity was detected with avidin, biotinylated horseradish peroxydase and 3,3'-Diaminobenzidine (DAB) (1B). 1A: negative control, incubated only with secondary antibody. Magnification 20×.
Violini et al. BMC Cell Biology 2009 10:29 doi:10.1186/1471-2121-10-29