Additional file 5.
Subcellular localization of EGFP tagged β-ENaC and mCherry-NEDD4L-C2(+)-C943A in response to ionomycin treatment in the presence of Ca2+. The movie, taken at 1 frame every 10 seconds, is of an A6 cell that stably expresses EGFP-β-ENaC, was transiently transfected with mCherry-NEDD4L-C2(+)-C943A, and was incubated in 1.97 mM Ca2+ and 10 μM ionomycin. It is the same A6 cell that is shown in Figure 5, panels K-O.
Format: MOV Size: 1.2MB Download file
Garrone et al. BMC Cell Biology 2009 10:26 doi:10.1186/1471-2121-10-26