Additional file 1.

EGFP-NEDD4L-C2(-) does not colocalize with markers of mitochondria, the endoplasmic reticulum (ER), the Golgi complex or lysosome. Confocal images of live A6 cells that were transiently transfected with EGFP-NEDD4L-C2(-) (A-L) and incubated in the presence of MitoTracker® Red (A-C), ER-Tracker™ Red (D-F), BODIPY® TR C5-ceramide complexed to BSA (Golgi marker) (G-I) or Lysotracker® Red (J-L). Blue and green channel overlay (A, D, G, J). Blue and red channel overlay (B, E, H, K). Blue, green and red channel overlay (C, F, I, L). Scale bars are equivalent to 10 μm.

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Garrone et al. BMC Cell Biology 2009 10:26   doi:10.1186/1471-2121-10-26