Real-time PCR of surfactant-associated protein mRNA expression in lung. Lung tissue was stored in the RNALater® buffer for extraction of total RNA. Real-time PCR was performed for quantification of mRNA levels for SP-A, SP-B and SP-C and normalized to the house-keeping gene RPL13A. To obtain ratios of normalized mRNA levels for each protein for the ASCFTR and control lung, samples were paired with respect to the day of sacrifice. The average results of triplicate samples are paired and calculated as percent of control. Results are expressed as percent change (in comparison to the control) and are mean ± SE of 5 pairs of animals for each protein. * The difference was considered significant at ≥ 50% decrease.
Gad et al. BMC Cell Biology 2009 10:24 doi:10.1186/1471-2121-10-24