Coimmunoprecipitation of mimitin with MAP1S. In HepG2 cells MAP1S with c-Myc tag and mimitin genes were overexpressed. The coimmunoprecipitation analysis was performed with ProFoundTM c-Myc Tag IP/Co-IP Kit. Proteins present in cells extracts and eluates were detected in Western blot with antibodies specific for Mimitin and specific for Myc tag in case of MAP1S.
Wegrzyn et al. BMC Cell Biology 2009 10:23 doi:10.1186/1471-2121-10-23