SPR analysis of peptide interactions. A) The ALP sequence between residues 152 and 246 with the ZM consensus motif marked in bold. Sequences of five overlapping peptides of ALP internal region are shown next, followed by the five deletion mutants and one point mutant. B) SPR sensograms showing that ALP peptide 3 interacts with the rod domain, whereas peptides 1, 2, 4 and 5 do not. R1-R4 was used as a ligand. C) R1-R4 interaction with ALP peptide 3 (used as a ligand) is concentration-dependent, but R2-R3 does not interact with peptide 3. D) Comparison between the binding of peptide 3 and its scrambled version containing the same amino acids in random order.
Klaavuniemi et al. BMC Cell Biology 2009 10:22 doi:10.1186/1471-2121-10-22