INA-UV induced apoptosis in SupT1 cells is mediated by caspases. A, B. SupT1 cells, pre-incubated or not with 40 μM ZVADfmk were treated with indicated amounts of INA, irradiated with UV and analyzed 24 h post treatment. A. Cells were stained with DiOC6 and analyzed by FACS. The percentage of the population presenting low staining (depolarized mitochondria) is presented. B. Cells were stained with Annexin V and analyzed by FACS. The percentage of the population presenting high staining (PS exposure) is presented. C. Cells were stained with FITC-VAD-FMK and analyzed by FACS. The percentage of the population presenting high staining (caspase activated) is presented. Data are mean ± S.D. Respresentative graphs of two independent experiments are presented. D. SupT1 cells were treated with indicated amounts of INA or doxorubicin. 24 hours upon treatment, the cells were lysed and the presence of PARP and/or cleaved PARP was assessed by Western analysis. Loading equivalence was assessed by Western blot analysis of GAPDH.
Viard et al. BMC Cell Biology 2009 10:21 doi:10.1186/1471-2121-10-21