INA-UV treatment induces apoptosis. INA-UV treatment of MCF7 cells induces mitochondrial membrane depolarization (DiOC6 staining), phosphatidyl serine exposure (Annexin V binding) and DNA fragmentation (TUNEL assay). MCF7 cells treated with increasing amounts of INA and irradiated with UV were stained with DiOC6 (A) or Annexin V (B). The percentage of the population with low DiOC6 staining (depolarized mitochondria) or high Annexin V staining (PS exposure on the cell surface) were determined by FACS analysis and plotted as a function of the INA used for the treatment. Data are mean ± S.D. Representative graphs from two independent experiments are presented. C. Histograms corresponding to the TUNEL staining of MCF7 cells treated with different amounts of INA and irradiated with UV.
Viard et al. BMC Cell Biology 2009 10:21 doi:10.1186/1471-2121-10-21