Inhibition of BMP mediated activation of BRE-Luc with specific blocking antibodies. C2C12BRE cells were treated as in Figure 1 with (A) 5 ng/ml BMP4, (B) 20 ng/ml BMP6 and (C) 5 ng/ml BMP9 with or without increasing concentrations of BMP4, BMP6 and BMP9 specific blocking antibodies respectively (D, E, F, G, H, I). C2C12BRE cells were treated as in Figure 1 with 5 ng/ml BMP4 (D), 20 ng/ml BMP6 (E), 2 ng/ml BMP9 (F), 50 ng/ml BMP2 (G), 10 ng/ml BMP7 (H) or 20 ng/ml BMP10 (I) in the presence or absence of 5 ng/ml BMP4 antibody, 2 ng/ml BMP6 antibody or 0.1 ng/ml BMP9 antibody. Luciferase activity was normalised to protein content and fold inductions relative to non-BMP treated samples are shown. Each point represents the mean ± SEM of three independent experiments performed in quadruplicate.
Herrera and Inman BMC Cell Biology 2009 10:20 doi:10.1186/1471-2121-10-20