Determination of the linear range of activation of BRE-Luc by BMPs. C2C12BRE cells were treated as in Figure 1 with increasing concentrations of (A) BMP4, (B) BMP6, (C) BMP7, (D) BMP9 and (E) BMP10. Luciferase activity was normalised to protein content. Relative luciferase units (RLU) following background subtraction are shown. Data shown is a representative experiment performed in quadruplicate ± SD.
Herrera and Inman BMC Cell Biology 2009 10:20 doi:10.1186/1471-2121-10-20