BMP-specific dose dependent induction of BRE-Luc activity in C2C12BRE cells. C2C12BRE cells were seeded overnight in 24 well plates at 2 × 104 cells per well and serum starved in DMEM containing 0.1% FCS for 7 hours. After serum starvation, cells were incubated without or with increasing concentrations of recombinant human (A) BMP2, (B) BMP4, (C) BMP6, (D) BMP7, (E) BMP9, (F) BMP10, (G) TGF-β1, (H) Nodal and (I) MIS. After 16 hours of treatment, BRE-luciferase activity was assessed by measuring luciferase activity in cell lysates. Luciferase activities were normalised to protein content and fold inductions relative to untreated samples were determined. Each point represents the mean ± SEM of three independent experiments performed in quadruplicate.
Herrera and Inman BMC Cell Biology 2009 10:20 doi:10.1186/1471-2121-10-20