Immuno-FISH on metaphase spreads from HAC containing cells lines in human and murine cells. The human HAC are grouped with their murine derivatives. HAC are stained with antibodies for CENP A, H3diK4, H3triK9 and H3phosphoS28 (green signal) and hybridized by FISH with the 17α probe (red signal). The chromosomes are counterstained in DAPI (blue). The small white inset shows a 3× enlargement of the HAC identified by the yellow arrows. To appreciate the amount of H3diK4, H3triK9 and H3phosphoS28 bound by the HAC, the FISH signal is not displayed in the corresponding white inset.
Moralli et al. BMC Cell Biology 2009 10:18 doi:10.1186/1471-2121-10-18