FISH on metaphase spreads from HAC containing cells lines in human and murine cells. Metaphase spreads from all HAC clones were hybridized to 17α DNA (green signal). The HAC in human cells (h) are grouped with the derivative HAC in murine cells (m). Chromosomes are counterstained by DAPI (blue). The yellow arrows denote the HAC. The inset shows DNA staining only, with HAC identified by the red arrow.
Moralli et al. BMC Cell Biology 2009 10:18 doi:10.1186/1471-2121-10-18