SPRI apparatus and spatial resolution. A) Schematic for SPR imaging instrument. Incident LED illumination is spatially filtered, collimated, and directed by mirrors through a SF-10 prism coupled to a commercially available cell environmental chamber designed for transmission and fluorescence microscopies. The reflected image is captured on a CCD camera. B) SPR images were generated with 470 nm and 630 nm incident light. Images shown are 1/16 of the total SPR field of view. Photolithographically patterned PDMS stamps with either 5 μm circle or 2 μm line features were placed directly on bare gold. Normalized intensity line profiles for 5 μm PDMS features compare lateral resolution using 470 nm and 630 nm incident light. Reflectivity line profiles for 2 μm PDMS lines compare reflectance intensity and dynamic range for 470 nm and 630 nm light. Pixel size is approximately 1 μm.
Peterson et al. BMC Cell Biology 2009 10:16 doi:10.1186/1471-2121-10-16