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Open Access Highly Accessed Methodology article

Surface plasmon resonance imaging of cells and surface-associated fibronectin

Alexander W Peterson1*, Michael Halter1, Alessandro Tona2, Kiran Bhadriraju2 and Anne L Plant1

Author Affiliations

1 Cell and Tissue Measurements Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA

2 SAIC, Arlington, VA, USA

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BMC Cell Biology 2009, 10:16  doi:10.1186/1471-2121-10-16

Published: 26 February 2009

Additional files

Additional file 1:

Quantifying protein deposition at cell periphery. Movie showing image analysis procedure used to quantify protein deposition at the cell periphery after 24 h in culture by dilating the cell contours to extract protein coverage versus distance-from-cell edge. Also, shows image analysis for low (<15%) and high (>25%) cell density regions.

Format: MOV Size: 1.4MB Download file

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