Analysis of the expression of various truncated EGFP-RIG1 fusion proteins. (A) Schematic diagram of RIG1 expression vectors that express wild type or various truncated RIG1 proteins. (B) Western blot analysis of the truncated EGFP-tagged RIG1 fusion proteins. HtTA cells plated in 6-cm dishes were transiently transfected with 1.5 μg of indicated expression vectors for 24 h. Fifty μg of cellular proteins were resolved by 15% SDS-PAGE. Expression of RIG1 variants was detected using anti-myc or anti-EGFP antibodies. (C) Subcellular localization of EGFP-tagged RIG1 variants. Cells were transiently transfected with indicated expression vectors for 18 h. Cells were fixed and analyzed with a laser scanning confocal microscope. Scale bar: 5 μm; wt: wild type.
Tsai et al. BMC Cell Biology 2009 10:15 doi:10.1186/1471-2121-10-15