Additional file 9.
PCR confirmation of hspc300 disruption. Vegetative wild type NC4A2 and IR55 hspc300- cells were screened by PCR using primers upstream and downstream of the knockout construct as described in Materials & Methods. The wild type band is 1.7 kbp long and the hspc300 gene disruption band is 3 kbp long (1.7 kbp and 1.3 kbp for the blasticidin insert).
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Pollitt and Insall BMC Cell Biology 2009 10:13 doi:10.1186/1471-2121-10-13