Visualisation of Mcm4-Mcm4 interactions by BiFC. A. BiFC, DNA and DIC images of exponentially-growing mcm4-VN173/mcm4-VC155 diploid cells. Bar = 10 μm. B. Chromatin-associated BiFC signals in detergent-extracted mcm4-VN173/mcm4-VC155 cells. Bar = 10 μm. C. Quantitation of nuclear and chromatin-bound BiFC signals in mcm4-VN173/mcm4-VC155 cells. At least 100 cells were counted for each data point.
Akman and MacNeill BMC Cell Biology 2009 10:12 doi:10.1186/1471-2121-10-12