Visualisation of Psf1-Mcm4 interactions by BiFC. A. Haploid wild-type, mcm4-VC155, psf1-VN173 and psf1-VN173 mcm4-VC155 cells. B. Chromatin-associated BiFC signal in detergent-extracted psf1-VN173 mcm4-VC155 cells. C. Quantitation of nuclear (detergent-extractable) and chromatin-bound (detergent-extraction resistant) BiFC signals in entire population, in uninucleate cells and in binucleate (S-phase) cells. D. Quantitation (as above) of nuclear and chromatin-bound BiFC signals in exponentially-growing cells, and in cells treated with 12 mM hydroxyurea for 2 and 4 hours. At least 100 cells were counted for each data point.
Akman and MacNeill BMC Cell Biology 2009 10:12 doi:10.1186/1471-2121-10-12