Sec61β protein and Gurken mRNA in ovaries from wild type and sec61βP1 germline clones. A) Ovaries were dissected from flies and lysed in buffer containing 2% SDS. Western blotting was performed using Sec61β antibody. A cross-reactive band serves as a loading control. B) Schematic representation of a stage 9–10 egg chamber with the oocyte abutting the 15 nurse cells and surrounded by a layer of somatic follicle cells. The dorsal/anterior corner is depicted in dark red. C-D) Drosophila egg chambers were processed for gurken RNA in-situ hybridization in wild type oocytes, C) and in oocytes mutant for Sec61β, D). n represents the oocyte nucleus.
Kelkar and Dobberstein BMC Cell Biology 2009 10:11 doi:10.1186/1471-2121-10-11