LN332 and Integrin α3 inhibition decrease matrix and keratin 10 production. Wound tissues were collected at wound closure and sections made. (A) Representative hematoxylin and eosin-stained sections show that in the LN332 and integrin α3 antibody-treated mice the epidermis is morphologically less differentiated, the basal keratinocytes are not well aligned, the reticular ridges not well formed, and the number of layers of keratinocytes is higher. (B) Immunolabeling for Collagen IV, a marker for basement membrane development, shows that the LN332 antibody causes a dramatic decrease in basement membrane deposition and integrin α3 antibody results in a basement membrane that contains many densities that contributes to irregularity of Collagen IV deposition. Solid lines demark the apical surface of the epidermis and arrowheads point to basement membrane). (C) Immunolabeling for keratin 10, a marker for keratinocyte differentiation, shows that the basal layers of the epidermis are disorganized and undifferentiated when LN332 and integrin α3 antibodies are applied to the wounds. Dashed lines delineate the basal surface of the epidermis and the vertical lines delineate the thickness of the epidermis.
Liu et al. BMC Cell Biology 2009 10:1 doi:10.1186/1471-2121-10-1