The C-terminal domain of C/EBPβ interacts with Elk-1. Radiolabeled Elk-1 was prepared by in vitro transcription and translation of CMV-Elk-1 expression construct and then incubated with equivalent amounts of GST, GST-p35-C/EBPβ, or GST-p20-C/EBPβ protein immobilized on glutathione-Sepharose. Proteins eluted from the GST-p20-C/EBPβ beads (lane 2), the GST-p35-C/EBPβ beads (lane 3) or GST beads (lanes 4) were resolved on an SDS-10% polyacrylamide gel and detected by autoradiography. Lane 1 contains one-half of the amount of the radiolabeled proteins initially mixed with the beads.
Hanlon et al. BMC Cell Biology 2000 1:2 doi:10.1186/1471-2121-1-2