Elk-1 binds to GST-p35-C/EBPβ in vitro. Radiolabeled Elk-1 was prepared by in vitro transcription and translation of CMV-Elk-1 expression construct and then incubated with equivalent amounts of GST or GST-p35-C/EBPβ protein immobilized on glutathione-Sepharose. Proteins eluted from the GST-p35-C/EBPβ beads (lanes 2) or GST beads (lanes 3) by boiling in Laemmli sample buffer were resolved on an SDS-10% polyacrylamide gel and detected by autoradiography. Lane 1 contains one-fourth of the amount of the radiolabeled proteins initially mixed with the beads.
Hanlon et al. BMC Cell Biology 2000 1:2 doi:10.1186/1471-2121-1-2