This article is part of the supplement: Italian Society of Bioinformatics (BITS): Annual Meeting 2007
Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation
1 Lab. Viral Oncogenesis and Immunotherapies & AIDS Reference Center, Department of Experimental Oncology, Istituto Nazionale Tumori “Fond. G. Pascale”, 80131 Napoli, Italy
2 Immunogenetics Section, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1502, USA
3 Department of Cell Biolology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy
4 Institute of Human Virology, University of Maryland School of Medicine, University of Marlyand, Baltimore, MD 21201, USA
BMC Bioinformatics 2008, 9(Suppl 2):S5 doi:10.1186/1471-2105-9-S2-S5Published: 26 March 2008
Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines.
The analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and “simplified” experimental procedure.
The results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.