Table 2

Conservation of DNA binding residues

Residue

% Freq.

Other residues


162-Ser

59

Ala 14%, Gln 12%

164-Met

20

Val 62%

165-Gly

99

168-Ala

100

169-Arg

99

172-Ser

99

176-Arg

99

196-Arg

99

197-Met

47

Glu 42%

198-Lys

98

199-Ile

74

Val 25%

200-Gly

99

207-Glu*

99

208-Thr

90

211-Gly

100

212-Gly

99

213-Asn

52

Arg 31%

226-Arg*

97

243-Arg*

56

Lys 41%

245-Lys*

96

280-Lys*

30

Glu 32%, Asp 16%

282-Lys*

19

Gly 36%, Asp 29%

286-Lys*

93

302-Lys*

46

Arg 47%


The first column lists E. coli RecA residues directly involved in DNA binding and those residues proposed (*) to interact with DNA [29]. The "% Freq." column reports the percent frequency of the indicated amino acid among 300 RecA homologs. The last column shows the percent frequency of other residues at that position of the alignment. See text for a description of conservation at these positions among eukaryotic and archaeal RecA homologs.

Roca et al. BMC Bioinformatics 2008 9:554   doi:10.1186/1471-2105-9-554

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