Table 1

Accuracy of scoring the metaphase and telophase phenotype

Metaphase

Telophase

# positive cells (Observer 1)

35

25

# positive cells (Observer 2)

40

38

Mean # positive cells (Observers 1&2)

37.5

31.5

# positive cells (CellProfiler)

16

20

# cells called positive by CellProfiler but not by Observers

0

1

# cells called positive by Observers but not by CellProfiler

21.5

(37.5-16)

11.5

(31.5-20)

false positive rate

0%

(0/16)

5%

(1/20)

false negative rate

57%

(21.5/37.5)

37%

(11.5/31.5)

Ten randomly selected images from the screen (~5000 cells total) were scored by CellProfiler Analyst and by two observers.

Jones et al. BMC Bioinformatics 2008 9:482   doi:10.1186/1471-2105-9-482