The widerborst RNA interference metaphase arrest phenotype is confirmed in Drosophila and human cells. (a) A sampling of metaphase nuclei produced by widerborst knockdown in Drosophila Kc167 cells (top). Scale bar = 5 μm. Quantitative confirmation of the increased percentage of metaphase nuclei, as scored by two blinded observers (bottom). Bars are starred if p < 0.05. Error bars = SEM. Controls are nearby spots lacking dsRNA. (b) A sampling of metaphase nuclei produced by PPP2R5E knockdown in human HT29 cells (top). Scale bar = 10 μm. Quantitative confirmation (bottom). Bars are starred if p < 0.001. Error bars = SEM. The infection rate is the ratio of the number of cells with/without puromycin, the selection agent. The control shRNA is FLJ25006 (NMid = NM_144610).
Jones et al. BMC Bioinformatics 2008 9:482 doi:10.1186/1471-2105-9-482