Screen revealed RNA interference samples enriched in telophase- and metaphase-stage nuclei. (a) Composite images of handpicked Drosophila Kc167 nuclei that were measured to guide feature selection and provide the starting point for developing the gates. Other nuclei at the edges of each sub-panel of the composite image were excluded from analysis. (b) A random sample of automatically scored nuclei from the cell microarrays. The scored cell is in the center of each panel unless the cell was near the edge of the image. Scale bars = 5 μm. (c) Genes whose dsRNAs significantly increase the proportion of cells in telophase (first four rows) or metaphase (last row). We excluded one gene (CG3245) that originally scored as a telophase hit because visual examination revealed that one image contained debris that disrupted proper analysis. Note that CG8878 should not be considered telophase-specific (see text). The fourth column shows the fold-change in the percentage of cells with each DNA content (2N, 4N, or 8N) and the fold-change in the total cell count overall, with significant differences from all genes tested on the array marked with a star. See Materials and Methods section for details on this image-based analysis of the cell cycle distribution. The fifth column indicates the fold-enrichment for the percentage of cells that were phospho-histone H3-positive, using the mean intensity of staining in the nucleus, based on the average of two replicates. References cited in the last column: A , B , C , D , E , F , G .
Jones et al. BMC Bioinformatics 2008 9:482 doi:10.1186/1471-2105-9-482