Relationships among data can be explored. Data points representing images with high nuclear area (averaged over the cells in the image) and high DNA intensity (averaged over the cells in the image) are highlighted in blue by brushing the scatterplot shown in (a). Immediately, the corresponding points appear blue in the other open scatterplot (b), allowing the relationship between all of the plotted features to be examined. As well, a DNA content histogram (c) shows individual cell data from the selected image data points (blue), relative to all cells in the experiment (red). In this case, the selected blue data points indeed have an unusual cell cycle distribution (fewer 2N cells relative to 4N cells) as compared to all cells in the experiment. Finally, the sample names (d, "Hairpin" column, for this particular RNA interference experiment) corresponding to the selected blue data points can be displayed in a table to see which samples are present in the selected points. The first two columns of the table show other information about those data points based on the axes of the scatterplot shown in (a). The "ImageNumber" and "well" columns provide additional information about the samples the researcher is investigating.
Jones et al. BMC Bioinformatics 2008 9:482 doi:10.1186/1471-2105-9-482