Additional File 1.

Signal over noise decays with sequencing cycle number. Histograms of the raw fluorescence intensities are shown for cycles 5, 15, 25, and 35. The separation between signal and noise is increasingly blurred and faster in the A and G channels than in the C and T channels. Red lines indicate a fit by a mixture of two Gaussians distributions with blue vertical bars indicating the mean and one standard deviation for the highest component of the mixture.

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Rougemont et al. BMC Bioinformatics 2008 9:431   doi:10.1186/1471-2105-9-431