BMC Bioinformatics

official impact factor 3.03

Open Access Methodology article

ReRep: Computational detection of repetitive sequences in genome survey sequences (GSS)

Thomas D Otto1,2*, Leonardo HF Gomes1,3, Marcelo Alves-Ferreira1, Antonio B de Miranda1 and Wim M Degrave1

Author Affiliations

1 Laboratory for Functional Genomics and Bioinformatics, IOC, Fiocruz, Rio de Janeiro, Brazil

2 Fundação Ataulpho de Paiva, Rio de Janeiro, Brazil

3 Medicine Faculty, UFRJ, Rio de Janeiro, Brazil

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BMC Bioinformatics 2008, 9:366 doi:10.1186/1471-2105-9-366

Published: 9 September 2008

Additional files

Additional file 1:

Sequences and Sequence Landscapes of the PRS. The DNA sequence and SL of PRS_1 – PRS_5 is given, using l = 400.

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Open Data

Additional file 2:

PCR reaction for the elements PRS_1. Column MW: 1 kb marker plus DNA Ladder. Size of the bands is indicated on the left. Column 1: amplification with primers: TTGTAAAACGACGGCCAGTG and CACACAGGAAACAGCTATGAC.

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Open Data

Additional file 3:

Multiple alignment for PRS_1. Multiple alignment of read GenBank: EI185111 and read GenBank: EI185194, representing PRS_1.

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Additional file 4:

Multiple alignment of the Tan-PRS_1 elements. The tandem elements of PRS_1 were extracted from the original GSS. Elements A1-A11 were obtained from GenBank: EI185111; elements B1-B10 from GenBank: EI185194.

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Additional file 5:

Table of repeats of E. coli K12. The name, the number of copies and the size of the repeats that are present in the E. coli K12 genome are listed, with indication, which of those are detected by ReRep analysis, with the corresponding parameter values of l and t.

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Open Data

Additional file 6:

FASTA file of repeats of E. coli K12.

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