Methodology article
A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
-
* Corresponding authors: Michele Guescini michele.guescini@uniurb.it - Vilberto Stocchi vilberto.stocchi@uniurb.it
- † Equal contributors
1 Istituto di Ricerca sull'Attività Motoria, Università degli Studi di Urbino "Carlo Bo", Via I Maggetti, 26/2 - 61029 Urbino, Italy
2 Istituto di Chimica Biologica "G. Fornaini", Università degli Studi di Urbino "Carlo Bo", Via Saffi, 2 - 61029 Urbino, Italy
3 Istituto di Biomatematica, Università degli Studi di Urbino "Carlo Bo", Campus Scientifico Sogesta, Loc Crocicchia, - 61029 Urbino, Italy
BMC Bioinformatics 2008, 9:326 doi:10.1186/1471-2105-9-326
Published: 30 July 2008Additional files
Additional file 1:
Windows Excel file containing PCR readouts and non-linear fittings.
Format: XLS Size: 578KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
Windows Word file containing first and second derivative of Richards equation and the mathematical formulas for obtaining the coordinate of the Cy0 point.
Format: DOC Size: 80KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 3:
Windows Excel file containing the Ct Cp Cy0 SCF Log10 SCF elaborations.
Format: XLS Size: 619KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 4:
Windows Excel file containing the results obtained with the SCF approach based on a previous study by Rutledge 2004.
Format: XLS Size: 120KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 5:
Windows Excel file containing the results obtained with the SCF approach based on a previous study by Rutledge 2004 in presence of IgG.
Format: XLS Size: 80KB Download file
This file can be viewed with: Microsoft Excel Viewer