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Open Access Highly Accessed Research article

Prediction of enzyme function based on 3D templates of evolutionarily important amino acids

David M Kristensen12, R Matthew Ward12, Andreas Martin Lisewski1, Serkan Erdin1, Brian Y Chen3, Viacheslav Y Fofanov4, Marek Kimmel4, Lydia E Kavraki235 and Olivier Lichtarge12*

Author Affiliations

1 Department of Molecular and Human Genetics, Biophysics, Baylor College of Medicine, Houston, TX 77030, USA

2 Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA

3 Department of Computer Science, Rice University, Houston, Texas 77030, USA

4 Department of Statistics, Rice University, Houston, Texas 77030, USA

5 Department of Bioengineering, Rice University, Houston, Texas 77030, USA

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BMC Bioinformatics 2008, 9:17  doi:10.1186/1471-2105-9-17

Published: 11 January 2008

Abstract

Background

Structural genomics projects such as the Protein Structure Initiative (PSI) yield many new structures, but often these have no known molecular functions. One approach to recover this information is to use 3D templates – structure-function motifs that consist of a few functionally critical amino acids and may suggest functional similarity when geometrically matched to other structures. Since experimentally determined functional sites are not common enough to define 3D templates on a large scale, this work tests a computational strategy to select relevant residues for 3D templates.

Results

Based on evolutionary information and heuristics, an Evolutionary Trace Annotation (ETA) pipeline built templates for 98 enzymes, half taken from the PSI, and sought matches in a non-redundant structure database. On average each template matched 2.7 distinct proteins, of which 2.0 share the first three Enzyme Commission digits as the template's enzyme of origin. In many cases (61%) a single most likely function could be predicted as the annotation with the most matches, and in these cases such a plurality vote identified the correct function with 87% accuracy. ETA was also found to be complementary to sequence homology-based annotations. When matches are required to both geometrically match the 3D template and to be sequence homologs found by BLAST or PSI-BLAST, the annotation accuracy is greater than either method alone, especially in the region of lower sequence identity where homology-based annotations are least reliable.

Conclusion

These data suggest that knowledge of evolutionarily important residues improves functional annotation among distant enzyme homologs. Since, unlike other 3D template approaches, the ETA method bypasses the need for experimental knowledge of the catalytic mechanism, it should prove a useful, large scale, and general adjunct to combine with other methods to decipher protein function in the structural proteome.