BMC Bioinformatics

official impact factor 3.03

Open Access Research article

A structural analysis of in vitro catalytic activities of hammerhead ribozymes

Yu Shao, Susan Wu, Chi Y Chan, Jessie R Klapper, Erasmus Schneider and Ye Ding*

Author Affiliations

Wadsworth Center, New York State Department of Health, 150 New Scotland Avenue, Albany, NY 12208, USA

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BMC Bioinformatics 2007, 8:469 doi:10.1186/1471-2105-8-469

Published: 30 November 2007

Additional files

Additional File 1:

A representative gel. In-vitro transcribed and purified ribozymes were analyzed by denaturing gel electrophoresis. The figure shows a representative gel with four different ribozymes.

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Additional File 2:

Sequences of hammerhead ribozymes. The table lists sequences of 15 hammerhead ribozymes targeted to the BCRP mRNA.

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Additional File 3:

Primer sequences for RT-PCR amplification. The table lists primer sequences for RT-PCR amplification of the ribozymes for quantifying cleavage reaction.

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Open Data