Table 2 |
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Kinetic constants for triose phosphate isomerases (TPI) from 12 different organisms with glyceraldehyde-3-phosphate as substrate |
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| Organism |
Km (mM)* |
kcat (105 min-1) |
kcat/Km (107 M-1s-1) |
SwissProt ID Sequence identity to 1r2rA |
Conditions, Reference |
|
|
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| Trypanosoma brucei |
0.46 (0.25–0.46) |
3.1 (2.6–3.7) |
1.120 |
TPIS_TRYBB 53% |
20°C, 100 mM, pH 7.4 [56] |
| Trypanosoma cruzi |
0.45 |
2.7 |
1.000 |
TPIS_TRYCR 53% |
20°C, 100 mM, pH 7.4 [57] |
| Oryctolagus cuniculus muscle |
0.43 (0.32–0.43) |
1.86 (1.9–5.1) |
0.721 |
TPIS_RABIT 100% |
30°C, 50 mM, pH 7.6 [56] |
| Gallus gallus muscle |
0.47 |
2.56 |
0.915 |
TPIS_CHICK 89% |
30°C, 100 mM, pH 7.4 [58] |
| Saccharomyces cerevisiae |
1.22 (0.62–1.5) |
7.9 (1.4–10)) |
1.080 |
TPIS_YEAST 52% |
25°C, 100 mM, pH 7.6 [59] |
| Leishmania mexicana |
0.41 (0.30–0.41) |
2.52 (4.3–2.5) |
1.020 |
TPIS_LEIME 52% |
25°C, 100 mM, pH 7.6 [60] |
| Plasmodium falciparum |
0.35 |
2.68 |
1.280 |
TPIS_PLAFA 42% |
30°C, 50 mM, pH 7.9 [56] |
| Vibrio marinus |
1.9 |
4.2 |
0.368 (0.515)# |
TPIS_VIBMA 41% |
10°C, 100 mM, pH 7.6 [61] |
| Escherichia coli |
1.03 |
5.4 |
0.874 |
TPIS_ECOLI 45% |
25°C, 100 mM, pH 7.6 [61] |
| Homo Sapiens |
0.49 |
2.7 |
0.918 |
TPIS_HUMAN 98% |
25°C, 100 mM, pH 7.6 [62] |
| Giardia lamblia |
0.53 |
2.9 |
0.912 |
TPI1_GIALA 45% |
25°C, 100 mM, pH 7.4 [63] |
| Spinacia oleracea |
0.68 |
2.7 |
0.662 |
TPIC_SPIOL 60% |
25°C, 100 mM, pH 7.5 [64] |
|
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* The range of values extracted from BRENDA is given in brackets. # The value after correction to 25°C obtained by multiplying by 1.4 (the ratio of water viscosity at 10°C compared to 25°C). |
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Gabdoulline et al. BMC Bioinformatics 2007 8:373 doi:10.1186/1471-2105-8-373 |
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