Figure 5.

Effect of palmitate on stearoyl-CoA desaturase (SCD) measured by western blotting. (A) SCD was downregulated in the palmitate (0.7 mM) cultures as compared to the oleate (0.7 mM) and control cultures. (B) Co-supplementation of oleate (0.3 mM) with palmitate (0.4 mM) prevented the downregulation of SCD. (C) Co-supplementing palmitate (0.4 mM) with oleate (0.3 mM) decreased LDH release significantly, P < 0.01 (t-test). P: treated with 0.7 mM palmitate for 48 hours, PO: treated with 0.4 mM palmitate plus 0.3 mM oleate for 48 hours. Data expressed as average +/- SD from three independent experiments, * significantly lower than palmitate, p < 0.01 by t-test.