Table 4 

Resolution of each calculation model 

Relative concentrations 
ΔCT 
E^{Ct} 
(Savrg E)^{Ct} 
(Pavrg E)^{Ct} 
LR N_{0} 
Sigmoid 
Exponential 









tvalue 
pvalue 
tvalue 
pvalue 
tvalue 
pvalue 
tvalue 
pvalue 
tvalue 
pvalue 
tvalue 
pvalue 
tvalue 
pvalue 



1 – 0.1 
122.7 
0.000 
18.6 
0.000 
141.7 
0.000 
141.3 
0.000 
18.9 
0.000 
29.1 
0.000 
18.2 
0.000 
0.1 – 0.02 
7.3 
0.000 
1.9 
0.030 
12.6 
0.000 
12.6 
0.000 
3.5 
0.000 
4.4 
N/A 
0.8 
0.225 
0.02 – 0.01 
0.7 
0.232 
0.4 
0.360 
1.6 
0.052 
1.6 
0.052 
0.9 
0.190 
6.1 
0.000 
0.4 
N/A 
0.01 – 0.001 
0.5 
0.325 
0.3 
0.375 
1.3 
0.105 
1.2 
0.107 
0.5 
0.306 
0.1 
0.478 
2.7 
0.004 


A ANOVA coupled ttest was performed to determine which dilutions are statistically different from one another. The comparison of adjacent dilutions are shown. A pvalue higher than 0.05 indicate that the model is unable to discriminate between the two adjacent dilutions. N/A stands for not available and indicate that the expected higher concentration of the comparison was in fact calculated to be lower from the qPCR results. 

Karlen et al. BMC Bioinformatics 2007 8:131 doi:10.1186/147121058131 