Table 4

Resolution of each calculation model

Relative concentrations

ΔCT

ECt

(Savrg E)Ct

(Pavrg E)Ct

LR N0

Sigmoid

Exponential








t-value

p-value

t-value

p-value

t-value

p-value

t-value

p-value

t-value

p-value

t-value

p-value

t-value

p-value


1 – 0.1

122.7

0.000

18.6

0.000

141.7

0.000

141.3

0.000

18.9

0.000

29.1

0.000

18.2

0.000

0.1 – 0.02

7.3

0.000

1.9

0.030

12.6

0.000

12.6

0.000

3.5

0.000

-4.4

N/A

0.8

0.225

0.02 – 0.01

0.7

0.232

0.4

0.360

1.6

0.052

1.6

0.052

0.9

0.190

6.1

0.000

-0.4

N/A

0.01 – 0.001

0.5

0.325

0.3

0.375

1.3

0.105

1.2

0.107

0.5

0.306

0.1

0.478

2.7

0.004


A ANOVA coupled t-test was performed to determine which dilutions are statistically different from one another. The comparison of adjacent dilutions are shown. A p-value higher than 0.05 indicate that the model is unable to discriminate between the two adjacent dilutions. N/A stands for not available and indicate that the expected higher concentration of the comparison was in fact calculated to be lower from the qPCR results.

Karlen et al. BMC Bioinformatics 2007 8:131   doi:10.1186/1471-2105-8-131

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