Table 3

Models for the use of single reaction efficiencies

Grouping of individual efficiencies for average determination


Model

Amplicon (7)

cDNA (4)

Dilutions (5)

Replicates (5)

Reactions (700)


(PavrgE)Ct *

individual

pooled

pooled

pooled

100

(SavrgE)Ct

individual

individual

pooled

pooled

25

Ect

individual

individual

individual

pooled

5


*Each line indicates how single efficiency values are grouped in each of the calculation models. For instance, the (PavrgE)Ct model line indicates that one individual amplicon is chosen out of the 7 available ones, then efficiencies estimated from the different cDNA samples, dilutions and replicates are all pooled, leading to the determination of one efficiency value from 100 reactions. In the (SavrgE)Ct model, individual efficiency values are calculated for each cDNA sample and each amplicon averaging efficiency values from the 5 replicate reactions performed on the 5 dilutions (25 values overall).

Karlen et al. BMC Bioinformatics 2007 8:131   doi:10.1186/1471-2105-8-131

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