Resolution:
## Figure 2.
Measurement of the efficiency of a PCR reaction. A: Estimation of the efficiency using the Serial dilution (SerDil) method. Five
dilutions of a cDNA sample were amplified using the fibronectin (FN) amplicon. Each
dilution was analyzed with five replicates PCR reactions and each data point represents
one Ct value determined as in Figure 1B. Linear regression parameters and calculation of
the efficiency value are shown in the inserted textbox. B: Screenshot of the LinReg
PCR program analysis window, which allows the estimation of the efficiency value from
each set of amplification curves [13]. Data correspond to one of the reactions performed
from the undiluted sample used in part A. C: Comparison of the efficiency values obtained
using the Serial dilution and LinReg methods for the FN amplicon. Efficiency values
were determined from four independent cDNA samples using the Serial Dilution method
as in part A, or the LinReg method as in part B. For each sample, efficiency value
were either determined from one linear regression performed on 24 reactions altogether
(Serdil) and error bars calculated from the standard deviation on the slope as determined
from the linear regression method or the individual efficiency values determined from
each of the same 24 PCR reactions (LinReg) were averaged, and error bars represent
the standard deviation on the set of values.
Karlen |