Table 1

The sample real-time PCR data for analysis. In this data set, there two types of samples (treatment and control); two genes (reference and target); and four concentrations of each combination of gene and sample. For data quality control and ANCOVA analysis, the real-time PCR sample data set can be grouped in four groups according to the combination of sample and gene. The Control-Target combination effect was named group 1, Treatment-Target group 2, Control-Reference group 3 and Treatment-Reference group 4.

Replicate

Sample

Gene

Concentration

Ct

Group (Class)


1

Control

Target

10

23.1102

1

2

Control

Target

10

22.9003

1

3

Control

Target

10

22.8972

1

1

Control

Target

2

26.5801

1

2

Control

Target

2

26.2139

1

3

Control

Target

2

26.0606

1

1

Control

Target

0.4

28.1125

1

2

Control

Target

0.4

28.1899

1

3

Control

Target

0.4

27.5949

1

1

Control

Target

0.08

30.2772

1

2

Control

Target

0.08

30.4667

1

3

Control

Target

0.08

30.7571

1

1

Treatment

Target

10

21.7813

2

2

Treatment

Target

10

21.7564

2

3

Treatment

Target

10

21.641

2

1

Treatment

Target

2

23.7965

2

2

Treatment

Target

2

23.7571

2

3

Treatment

Target

2

23.724

2

1

Treatment

Target

0.4

26.3794

2

2

Treatment

Target

0.4

26.2542

2

3

Treatment

Target

0.4

25.9621

2

1

Treatment

Target

0.08

28.5479

2

2

Treatment

Target

0.08

28.3894

2

3

Treatment

Target

0.08

28.3416

2

1

Control

Reference

10

19.7415

3

2

Control

Reference

10

19.494

3

3

Control

Reference

10

19.3906

3

1

Control

Reference

2

21.9838

3

2

Control

Reference

2

22.4435

3

3

Control

Reference

2

22.57

3

1

Control

Reference

0.4

24.8109

3

2

Control

Reference

0.4

24.4327

3

3

Control

Reference

0.4

24.2342

3

1

Control

Reference

0.08

26.7319

3

2

Control

Reference

0.08

26.8206

3

3

Control

Reference

0.08

26.822

3

1

Treatment

Reference

10

18.4468

4

2

Treatment

Reference

10

18.8227

4

3

Treatment

Reference

10

18.3061

4

1

Treatment

Reference

2

21.2568

4

2

Treatment

Reference

2

21.0956

4

3

Treatment

Reference

2

20.8473

4

1

Treatment

Reference

0.4

23.2322

4

2

Treatment

Reference

0.4

22.9577

4

3

Treatment

Reference

0.4

23.2415

4

1

Treatment

Reference

0.08

25.4817

4

2

Treatment

Reference

0.08

25.608

4

3

Treatment

Reference

0.08

25.5675

4


Yuan et al. BMC Bioinformatics 2006 7:85   doi:10.1186/1471-2105-7-85

Open Data