Real-time PCR. (A) Theoretical plot of PCR cycle number against PCR product amount is depicted. Three phases can be observed for PCRs: exponential phase, linear phase and plateau phase. (B) shows a theoretical plot of PCR cycle number against logarithm PCR product amount. Panel (C) is the output of a serial dilution experiment from an ABI 7000 real-time PCR instrument.
Yuan et al. BMC Bioinformatics 2006 7:85 doi:10.1186/1471-2105-7-85