Steps in the design of MIPs: a) The software generates candidates for each target, based on the user specified criteria. b) The candidates are checked for their nonspecific binding with non targets and the host genome and nonspecific ones are eliminated and the specific ones are selected for designing MIP probes. n = genome length; k = user specified target binding length of probe; Kmer = genome fragment or oligo of length = k. (Numerical value of K = k).
Thiyagarajan et al. BMC Bioinformatics 2006 7:500 doi:10.1186/1471-2105-7-500