Functions of the Examiner module. PET extraction results can be viewed at various levels (library, plate, well, and nucleotide sequence). A. The library view of PET extraction result. The 4 libraries analyzed in this study were highlighted. The numbers of total PETs, unique PETs, and high quality sequence reads used for PET extraction are shown. B. The plate view of PET extraction results. Individual plates (plate ID) and the number of quality sequences, total PETs and cumulative PETs are shown in table format as well as graphic bar display. C. The 384-well view of PET extraction results. The digit in each well stands for the number of PETs generated from the sequence in that well. Each well is also color coded for 4 different categories based on the number of PETs produced. The 4 categories are summarized at the top panel of the table. D. Individual sequence view of PET extraction results. A sequence was dissected into spacer sequences and the putative PET sequences in between two adjacent spacers. The spacer sequences are in black and with plain background, and the sequences in between spacers are highlighted in orange color. The good PET sequences are in blue, and the bad sequences in red. The sequence segments are further tabulated with detailed information regarding the position and the length of each segment.
Chiu et al. BMC Bioinformatics 2006 7:390 doi:10.1186/1471-2105-7-390