Figure 1.

The layout of a microarray scanner calibration slide. A: Cy5 block; B: Cy3 block. There are 384 (32 by 12) spots for each of the Cy5 or Cy3 block. Series 1 to 28 correspond to two-fold dilutions, each of which has 12 repeats. Series 29 to 31 are blanks and series 32 is the positioning marker.