Research article

Cyclic nucleotide binding proteins in the Arabidopsis thaliana and Oryza sativa genomes

Dave Bridges , Marie E Fraser and Greg BG Moorhead

Department of Biological Sciences, University of Calgary, 2500 University Dr. N.W. Calgary, AB, T2N 1N4 Canada

author email corresponding author email

BMC Bioinformatics 2005, 6:6doi:10.1186/1471-2105-6-6

Published:	11 January 2005

Abstract

Background

Cyclic nucleotides are ubiquitous intracellular messengers. Until recently, the roles of cyclic nucleotides in plant cells have proven difficult to uncover. With an understanding of the protein domains which can bind cyclic nucleotides (CNB and GAF domains) we scanned the completed genomes of the higher plants Arabidopsis thaliana (mustard weed) and Oryza sativa (rice) for the effectors of these signalling molecules.

Results

Our analysis found that several ion channels and a class of thioesterases constitute the possible cyclic nucleotide binding proteins in plants. Contrary to some reports, we found no biochemical or bioinformatic evidence for a plant cyclic nucleotide regulated protein kinase, suggesting that cyclic nucleotide functions in plants have evolved differently than in mammals.

Conclusion

This paper provides a molecular framework for the discussion of cyclic nucleotide function in plants, and resolves a longstanding debate about the presence of a cyclic nucleotide dependent kinase in plants.